Dual reporter system for in situ detection of plasmid transfer under aerobic and anaerobic conditions.
نویسندگان
چکیده
We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 76 13 شماره
صفحات -
تاریخ انتشار 2010